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Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

FromListen In - Bitesize Bio Webinar Audios


Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions

FromListen In - Bitesize Bio Webinar Audios

ratings:
Length:
39 minutes
Released:
Sep 1, 2022
Format:
Podcast episode

Description

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn:The difference and benefits of LASSO cloning over NGSHow LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomesHow this technology can impact our understanding of disease, drug discovery, precision medicine, and so much moreJoin Dr. Lorenzo Tosi as he describes LASSO cloning, a technique that will allow for the expression of an entire set of proteins from any organism in more rapid and cost-effective manner.The development of tools and techniques designed to sequence and understand genomes has advanced our understanding of the world at large. However, our ability to analyze the function of expressed molecules from these genomes is lagging. Even with the advent of next generation sequencing (NGS), which has changed the landscape of genomic sequencing through increased accuracy and speed, our experiments are still limited by the length of DNA targets being read.For more information visit: https://bitesizebio.com/webinar/long-adapter-single-stranded-oligonucleotide-lasso-probes-for-massively-multiplexed-cloning-of-kilobase-sized-genome-regions/
Released:
Sep 1, 2022
Format:
Podcast episode

Titles in the series (100)

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/