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Simultaneous Proteomics and Transcriptomics

Simultaneous Proteomics and Transcriptomics

FromListen In - Bitesize Bio Webinar Audios


Simultaneous Proteomics and Transcriptomics

FromListen In - Bitesize Bio Webinar Audios

ratings:
Length:
67 minutes
Released:
Sep 1, 2022
Format:
Podcast episode

Description

Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments.In this tutorial, you will find:-How you can run one scRNA-seq experiment with numerous protein markers in parallel-How you can increase your recovery of single cells (up to four times!) per experiment-How you can link phenotypes to transcriptomic profiles—with higher throughput methods!The last few years have seen the scale of single cell RNA-seq experiments increase exponentially, greatly enhancing our understanding of cell biology in development and disease. It is now feasible for researchers to characterize thousands of single cells in one experiment. However, important hallmarks of immune cell states are often not detected in scRNA-seq experiments. While lower throughput methods previously allowed researchers to link phenotypes or protein expression to transcriptomic profiles, the increase in scale of modern droplet-based methods resulted in a loss of such addressability.Here we describe two recently developed applications that utilize antibody-conjugated oligonucleotides to enhance existing scRNA-seq platforms.1. CITE-seq, which allows measurement of a potentially unlimited number of protein markers in parallel to transcriptomes.2. Cell Hashing, which enables sample multiplexing, robust multiplet detection and super-loading of scRNA-seq platforms, allowing confident recovery of 4 times as many single cells per experiment.Reagents for performing these assays, under the name TotalSeq™ are now available from BioLegend.For more information visit: https://bitesizebio.com/webinar/simultaneous-proteomics-and-transcriptomics-the-future-of-single-cell-analysis/
Released:
Sep 1, 2022
Format:
Podcast episode

Titles in the series (100)

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/