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Using qPCR to Validate Epigenetic Enrichment of Pathogen DNA

Using qPCR to Validate Epigenetic Enrichment of Pathogen DNA

FromListen In - Bitesize Bio Webinar Audios


Using qPCR to Validate Epigenetic Enrichment of Pathogen DNA

FromListen In - Bitesize Bio Webinar Audios

ratings:
Length:
58 minutes
Released:
Sep 1, 2022
Format:
Podcast episode

Description

qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analysis of rare events.In this webinar, you will learn:1. The epigenetic differences between microbial and human/animal genomes2. The use of restriction endonucleases to enrich for either pathogen genomes or the human genome from complex populations.3. How the use of enrichment and concentration can improve qPCR sensitivity4. The use of qPCR to validate enrichment from complex samples5. The use of NGS to validate pathogen enrichment from complex samplesJoin Dr. Allyn Forsyth as he describes how successful NGS analysis of complex microbiome samples can lead to the development of non-invasive colorectal cancer screening, as well as monitoring of disease states within other cancers and Alzheimer’s Disease.For more information visit: https://bitesizebio.com/webinar/validate-epigenetic-enrichment-pathogen-human-dna/
Released:
Sep 1, 2022
Format:
Podcast episode

Titles in the series (100)

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/