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Guide to CRISPR/Cas9 Delivery How to Maximize Editing

Guide to CRISPR/Cas9 Delivery How to Maximize Editing

FromListen In - Bitesize Bio Webinar Audios


Guide to CRISPR/Cas9 Delivery How to Maximize Editing

FromListen In - Bitesize Bio Webinar Audios

ratings:
Length:
61 minutes
Released:
Sep 1, 2022
Format:
Podcast episode

Description

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research.The main points in the webinar will include:How to design guide RNAs using online tools specific to the genome and application of interest.Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system.How you can create precise mutations using homology-directed repair, including template design and cleavage siteAn Essential Guide to CRISPR/Cas9 Editing EfficiencyWhile CRISPR/Cas9 editing is utilized in a wide variety of cell types, editing efficiency continues to pose a challenge to researchers. Join Dr. Allison Mayle, as she shares best practices for increasing CRISPR/Cas9 editing efficiency. Viewers will discover online tools to aid in CRISPR/Cas9 design and delivery and tips for optimizing your CRISPR/Cas9 experiments.In this webinar, Dr. Mayle will review the factors influencing genome editing, including target sequence selection and CRISPR delivery methods. A comparison of plasmid and viral vector delivery will be provided, as well as an introduction to DNA-free CRISPR/Cas9 ribonucleoprotein reagents. Additionally, Dr. Mayle will cover best practices for CRISPR knock-in mutagenesis via homology-directed repair (HDR) and applications available from new Cas9 protein variants.For more information visit: https://bitesizebio.com/webinar/guide-crisprcas9-delivery-maximize-editing-efficiency/
Released:
Sep 1, 2022
Format:
Podcast episode

Titles in the series (100)

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/