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Jan-Michael Peters Part 2: How do Cohesin and CTCF Fold DNA in Mammalian Genomes?
FromiBiology Podcast
Jan-Michael Peters Part 2: How do Cohesin and CTCF Fold DNA in Mammalian Genomes?
FromiBiology Podcast
ratings:
Length:
36 minutes
Released:
Nov 29, 2020
Format:
Podcast episode
Description
In his second talk, Peters presents evidence that cohesin is indeed necessary for genomic DNA to fold into loops. Long range DNA interactions such as loops can be detected using a technique called Hi-C. Using Hi-C, Peters shows that depleting cohesin removes DNA loops, while depleting the proteins that remove cohesin from DNA, results in bigger DNA loops. In addition, CTCF appears to recognize specific sequences that define the base of the loops. Incorporating all of this data, Peters describes a model in which DNA is extruded by cohesin to form a loop and the boundaries of the loop are determined by CTCF. Peters explains that many questions about the mechanism of DNA loop extrusion and its importance in cells remain to be answered.
Released:
Nov 29, 2020
Format:
Podcast episode
Titles in the series (97)
Jan-Michael Peters Part 1: Cohesin: Roles Beyond Sister Chromatid Cohesion?: It has been known for many years that the protein cohesin is necessary to join sister chromatids together before they are segregated during mitosis. Electron micrographs have shown that cohesin subunits form a ring complex which is thought to encircle... by iBiology Podcast