Mass Spectrometry: Techniques for Structural Characterization of Glycans

By Michael A. Madson

Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.

As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.

• Authored by an analytical chemist with more than 30 years of experience in research and industry
• Serves as a quick reference in mass spectral analysis and carbohydrates
• Includes more than 60 figures to aid in the retention of key concepts

• Language: English
• Publisher: Elsevier Science
• Release date: May 6, 2016
• ISBN: 9780128041352

Michael A. Madson

Mike Madson is CEO and Senior Research Scientist of BioLogistics, LLC, Iowa, USA

Book preview

Mass Spectrometry

Techniques for Structural Characterization of Glycans

Michael A. Madson

BioLogistics LLC, Ames, IA, USA

Table of Contents

Cover image

Title page

Copyright

Dedication

Mass Spectral Analysis of Carbohydrates

Abstract

1 Isolation Methods for MS Analysis

2 Component Monosaccharide Analysis by Acid Catalyzed Hydrolysis of Glycan and Qualitative Chromatographic Identification by HPLC

3 Identification of Glycoses Versus Glycitols by HPAEC-PAD Difference Chromatograms

4 The Methods for Preparing an Oligosaccharide Reducing Glycan from a Glycoprotein, Linked Through O-Glycosidic Linkage

Conclusions

References

Copyright

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Notices

Knowledge and best practice in this field are constantly changing. As new research and experience broaden our understanding, changes in research methods or professional practices, may become necessary.

Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information or methods described herein. In using such information or methods they should be mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility.

To the fullest extent of the law, neither the Publisher nor the authors, contributors, or editors, assume any liability for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein.

British Library Cataloguing-in-Publication Data

A catalogue record for this book is available from the British Library

Library of Congress Cataloging-in-Publication Data

A catalog record for this book is available from the Library of Congress

ISBN: 978-0-12-804129-1

For Information on all Elsevier publications visit our website at https://www.elsevier.com/

Dedication

We dedicate this book to my Lord and Savior Jesus the Christ, His Father & mine and the Holy Spirit who have spent countless hours with me in writing this book so that many others may grasp the power of these techniques and apply them to their quests in science. We hope chemistry and medicine will be advanced and lives will be saved or lengthened because of Their intervention into my life.

Mass Spectral Analysis of Carbohydrates

Abstract

We describe the use of mass spectral analysis for the qualitative analysis of glycans. We examine the spectra from Electrospray Ionization Mass Spectrometry (ESI MS), Matrix Assisted Laser Desorption Mass Spectrometry (MALDI MS) and Atmospheric Pressure Ionization Mass Spectrometry (API MS) of carbohydrates. The former are time-of-flight (TOF) instruments and the last a triple quadrapole instrument. We have one spectrum that is a single quadrapole instrument. We describe several methods that aid in the determination of oligosaccharide structure by MS. They are: N-