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Reliable quantification of fluorescence images

Reliable quantification of fluorescence images

FromListen In - Bitesize Bio Webinar Audios


Reliable quantification of fluorescence images

FromListen In - Bitesize Bio Webinar Audios

ratings:
Length:
40 minutes
Released:
Sep 1, 2022
Format:
Podcast episode

Description

In this webinar, you will learn:– How to overcome issues with stage drift and crosstalk between channels– How to obtain reliable images and reproducible quantification results– How to proceed with advanced image analysis after restoring imagesDuring fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimental trouble. Additional aberrations, such as stage drift, and crosstalk and chromatic aberration between channels, can also affect the imaging. Huygens image deconvolution and restoration is a proven method to revert these issues and recover a more realistic representation of the original object. After restoration of the image, you can proceed with the advanced Huygens analysis options for colocalization, object measurements, and tracking.This webinar will illustrate how you can make optimal use of the complete Huygens workflow to obtain reliable images and reproducible quantification results, focusing on the advanced analysis options offered by the Huygens software.For more information visit: https://bitesizebio.com/webinar/reliable-quantification-of-fluorescence-images-from-raw-data-to-reproducible-results/
Released:
Sep 1, 2022
Format:
Podcast episode

Titles in the series (100)

The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into the experience and expertise of leading researchers and commercial specialists to drive your research projects forward efficiently and productively. Listen In now! https://bitesizebio.com/listen-in/