Laboratory Investigation of Endocrine Disorders
By Michael R. Wills and Bill Havard
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Laboratory Investigation of Endocrine Disorders - Michael R. Wills
Kent
Preface to the Second Edition
The need for a Second Edition of this book within three years of publication of the First Edition confirms the need for a basic guide to the available endocrine laboratory tests. In this edition the original text has been revised and two additions have been made. A new chapter has been added which deals with disorders of calcium homeostasis and their investigation with particular reference to hypercalcaemia and hypocalcaemia. An Appendix has been included which contains a set of normal endocrine reference values and also the factors for the conversion of numerical values in ‘old’ or conventional units to those in the ‘new’ SI system. It is, however, important to recognize that reference values are affected by many variables. Among the latter the most important are the assay technique and the age and sex of the patient. The reference values given in the Appendix should, therefore, only be used as general guidelines and a diagnostic or therapeutic decision should be based on the reference values provided by the laboratory that has done the assay.
Michael R. Wills and Bill Havard
Preface to the First Edition
In the past decade there has been a vast increase in the number of laboratory tests that are available for the investigation of patients with endocrine disorders. This has occurred simultaneously with considerable developments in the understanding of the mechanisms involved in the pathogenesis of these disorders. Regrettably, all too often in the investigative process there has been some misuse of the laboratory tests as patients with endocrine disorders represent only a small proportion of the whole medical spectrum. The objective of this manual has been to provide senior clinical students and medical practitioners, in all grades and specialties, with a basic guide to the endocrine laboratory tests that are available; not only when to do which test but also precisely how to do it as well as recognizing its limitations. The manual should also be of value to the laboratory-based scientist, both the medical and the non-medical, as a standardized basis on which to provide advice to the laboratory user. In each section the basic physiology on which the investigations are based has been provided in order to give a rational basis for their usage. Although the normal range and reference values may vary slightly between individual laboratories, because of methodological variations, the tests and the protocols that we have described are all well established and non-controversial.
Michael R. Wills, Bill Havard and Peter J. Roylance
1
Thyroid disorders
Publisher Summary
This chapter discusses thyroid disorders. The thyroid gland secretes its principal hormones, thyroxine (T4) and tri-iodothyionine (T3) into the circulation where they are associated with two proteins that bind them specifically. T3 is the metabolically active form of thyroid hormone. Both T4 and T3 are largely bound to plasma proteins. It is the free hormone that is metabolically active. Changes in the concentration of the serum binding protein will lead to parallel changes in the serum values of total thyroid hormones, but the free thyroid hormone values remain constant. Hypothyroidism is the clinical condition resulting from decreased circulating concentrations of free (unbound) thyroid hormones. Hypothyroidism is commonly caused by primary thyroid failure when serum thyrotrophin concentration is high because of a lack of negative feedback at the pituitary level by thyroid hormones. It can also be secondary to pituitary failure of thyrotrophin secretion when serum thyrotrophin values are usually low or just within the normal range. Hyperthyroidism is the clinical condition resulting from increased circulating serum concentrations of free thyroid hormones. Symptoms include weight loss, heat intolerance, tachycardia, and lid retraction. The main causes of hyperthyroidism are Graves’ disease, toxic multinodular goitre, toxic nodule, painless thyroiditis, and trophoblastic thyroid stimulating hormone syndrome, but the condition can be induced by over-treatment with thyroid hormones.
Physiology
The thyroid gland secretes its principal hormones, thyroxine (T4) and tri-iodothyionine (T3) into the circulation where they are associated with two proteins (the thyroxine-binding proteins) that bind them specifically. By paper electrophoresis the proteins appear between the α1 and α2 globulins (thyroxine-binding globulin (TBG)) and ahead of the albumin (thyroxine-binding prealbumin (TBPA)); in addition, a minor fraction of the hormone is bound to albumin itself. In the bound form, thyroxine and tri-iodothyronine are distributed throughout the extracellular fluid and are measurable by chemical or immunochemical assay.
T3 is the metabolically active form of thyroid hormone. Nevertheless T3 measurements cannot be substituted for T4 measurements in the clinical assessment of thyroid function for a number of reasons. Eighty per cent of the circulating T3 is derived from de-iodination of T4 by peripheral tissues so that T3 is only an indirect reflection of thyroid secretion. Stress, starvation and systemic illness decrease the rate of de-iodination so that the serum T3 level may be normal in the sick hyperthyroid patient. Furthermore the suppression of thyroid-stimulating hormone (TSH) is more dependent on circulating levels of T4 than of T3 so that in iodine deficiency, for example, the TSH may be raised because the circulating concentration of T4 is low but the patient is euthyroid because the levels of T3 are normal. Similarly, adequate T3 replacement therapy of the hypothyroid patient may not suppress TSH secretion in contrast to adequate T4 replacement therapy.
Both T4 and T3 are largely bound to plasma proteins. In the case of T4 99.95% is bound and with T3 99.5%. It is the free hormone that is metabolically active. Changes in the concentration of the serum binding protein will lead to parallel changes in the serum values of total thyroid hormones, but the free thyroid hormone values remain constant.
The control of thyroid hormone secretion is complex. A tripeptide thyrotrophin-releasing hormone (TRH) is synthesized and stored in the median eminence of the hypothalamus. It is released into the portal veins of the pituitary stalk. In the anterior pituitary it promotes pulsatile release of the glycoprotein TSH. It also promotes the release of prolactin although the physiological significance of this is at present unknown.
Thyrotrophin, the circulating concentration of which shows some nyctohemeral variation, stimulates most metabolic processes in the thyroid from iodide uptake to release of thyroid hormones. The mechanism is probably that thyrotrophin binds to a receptor site on the thyroid cell membrane and activates adenyl cyclase. This leads to a cyclic adenosine monophosphate (AMP) mediated activation of one or more enzymes ultimately leading to an increased release of T4 and T3 into