Reduce, Refine, Replace. Developed over 50 years ago, the 3Rs provide a framework that urges researchers to think carefully about methods used to investigate scientific questions. In this episode of EASL Studio, we took a closer look at the third R, Replace. With the World Day for Laboratory Animals fast approaching, we discussed how to propel liver cell biology into the third dimension.What are the advantages of 3D cultures?Can liver cell types be studied in representative manner using 3D cultures?More importantly, which part of animal research can be replaced by 3D cell culture experiments?This and more in EASL Studio: YI Choice.Read more All EASL Studio Podcasts are available on EASL Campus.