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Transplantation-based screen uncovers inducers of muscle progenitor cell engraftment across vertebrate species
Transplantation-based screen uncovers inducers of muscle progenitor cell engraftment across vertebrate species
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Length:
20 minutes
Released:
Jan 14, 2023
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.01.13.523942v1?rss=1
Authors: Tavakoli, S., Garcia, V., Gahwiler, E., Adatto, I., Rangan, A., Messemer, K., Ashrafi Kakhki, S., Yang, S., Chan, V., Manning, M., Fotowat, H., Wagers, A., Zon, L.
Abstract:
Stem cell transplantation presents a potentially curative strategy for genetic disorders of skeletal muscle, but this approach is limited due to the deleterious effects of cell expansion in vitro and consequent poor engraftment efficiency. In an effort to overcome this limitation, we sought to identify molecular signals that enhance the myogenic activity of cultured muscle progenitors. Here, we report the development and application of a cross-species small molecule screening platform employing zebrafish and mouse, which enables rapid, direct evaluation of the effects of chemical compounds on the engraftment of transplanted muscle precursor cells. Using this system, we screened a library of bioactive lipids to identify those that could increase myogenic engraftment in vivo in zebrafish and mice. Two lipids, lysophosphatidic acid (LPA) and niflumic acid (NFA), are linked to activation of intracellular calcium ion flux, which showed conserved, dose-dependent and synergistic effects in promoting muscle engraftment across these vertebrate species.
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http://biorxiv.org/cgi/content/short/2023.01.13.523942v1?rss=1
Authors: Tavakoli, S., Garcia, V., Gahwiler, E., Adatto, I., Rangan, A., Messemer, K., Ashrafi Kakhki, S., Yang, S., Chan, V., Manning, M., Fotowat, H., Wagers, A., Zon, L.
Abstract:
Stem cell transplantation presents a potentially curative strategy for genetic disorders of skeletal muscle, but this approach is limited due to the deleterious effects of cell expansion in vitro and consequent poor engraftment efficiency. In an effort to overcome this limitation, we sought to identify molecular signals that enhance the myogenic activity of cultured muscle progenitors. Here, we report the development and application of a cross-species small molecule screening platform employing zebrafish and mouse, which enables rapid, direct evaluation of the effects of chemical compounds on the engraftment of transplanted muscle precursor cells. Using this system, we screened a library of bioactive lipids to identify those that could increase myogenic engraftment in vivo in zebrafish and mice. Two lipids, lysophosphatidic acid (LPA) and niflumic acid (NFA), are linked to activation of intracellular calcium ion flux, which showed conserved, dose-dependent and synergistic effects in promoting muscle engraftment across these vertebrate species.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Jan 14, 2023
Format:
Podcast episode
Titles in the series (100)
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