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Cas9-mediated tagging of endogenous loci using HITAG
Cas9-mediated tagging of endogenous loci using HITAG
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Length:
20 minutes
Released:
Nov 17, 2022
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.11.16.516691v1?rss=1
Authors: Kim, J., Kratz, A., Sheng, J., Zhang, L., Singh, B. K., Chavez, A.
Abstract:
To facilitate the interrogation of proteins at scale, we have developed High-throughput Insertion of Tags Across the Genome (HITAG). HITAG enables users to produce libraries of cells, each with a different protein of interest C-terminally tagged, to rapidly characterize protein function. To demonstrate the utility of HITAG, we fused mCherry to a set of 167 stress granule-associated proteins and characterized the factors which drive proteins to strongly accumulate within stress granules.
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Podcast created by Paper Player, LLC
http://biorxiv.org/cgi/content/short/2022.11.16.516691v1?rss=1
Authors: Kim, J., Kratz, A., Sheng, J., Zhang, L., Singh, B. K., Chavez, A.
Abstract:
To facilitate the interrogation of proteins at scale, we have developed High-throughput Insertion of Tags Across the Genome (HITAG). HITAG enables users to produce libraries of cells, each with a different protein of interest C-terminally tagged, to rapidly characterize protein function. To demonstrate the utility of HITAG, we fused mCherry to a set of 167 stress granule-associated proteins and characterized the factors which drive proteins to strongly accumulate within stress granules.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Nov 17, 2022
Format:
Podcast episode
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