The arrival in the US of plasmid-mediated resistance to colistin antibiotics, a last line of defense against many gram-negative bacilli, and a quorum sensing system in a eukaryote are topics of this episode hosted by Vincent, Michael, and Michele. Image: Etest used to determine the minimum inhibitory concentration of an antibiotic for a particular bacterium. Hosts: Vincent Racaniello, Michael Schmidt, and Michele Swanson.  Subscribe to TWiM (free) on iTunes, Stitcher, RSS, or by email. You can also listen on your mobile device with the Microbeworld app. Links for this episode E. coli with mcr-1 on a plasmid in the US (AAC) Emergence of plasmid mediated colistin resistance in China (The Lancet) Major breach in last line of defense (The Lancet) Resistance plasmid families in Enterobacteriaceae (AAC) EUCAST MIC breakpoints (ClinCalc) Role of ASM in microbial resistance one, two (bLogphase) Quorum sensing in fungi (Cell Host Microbe) Image credit Letters read on TWiM 129 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/m​icrobe ​and use the promo code MICROBE​. Send your microbiology questions and comments (email or mp3 file) to twim@microbe.tv