Sam Sternberg discusses his work on exploring and exploiting CRISPR-Cas immune systems, beginning as a graduate student with Jennifer Doudna, at a biotech start-up, and in his laboratory at Columbia University. Host: Vincent Racaniello Guest: Sam Sternberg  Become a Patron of TWiM! Links for this episode Sternberg Laboratory at Columbia Mechanism of substrate selection by Cas9 (RNA) DNA interrogation by Cas9 (Nature) Conformational control of DNA target cleavage by Cas9 (Nature) A Crack in Creation by Doudna and Sternberg What if we could rewrite the human genome? (YouTube) Sam Sternberg Music used on TWiM is composed and performed by Ronald Jenkees and used with permission. Send your microbiology questions and comments to twim@microbe.tv