Lightspeed Magazine, Issue 148 (September 2022): Lightspeed Magazine, #148
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LIGHTSPEED is a digital science fiction and fantasy magazine. In its pages, you will find science fiction: from near-future, sociological soft SF, to far-future, star-spanning hard SF--and fantasy: from epic fantasy, sword-and-sorcery, and contemporary urban tales, to magical realism, science-fantasy, and folktales.
Welcome to issue 148 of LIGHTSPEED! Our first SF story of the month might be one of our most timely. From writer MKRNYILGLD comes "The CRISPR Cookbook: A guide to biohacking your own abortion in a post-Roe world," and the title does a great job describing it. Be sure not to miss it. Our flash SF this month, "Civilian Assumptions," comes from Dominique Dickey, while our second piece of full-length SF is a wildly delightful story of bounty hunters, space travel, and strawberry lip gloss. Yes: lip gloss. Please enjoy R J Theodore's "The Application of Strawberry Lip Gloss in a Low-Gravity Environment." Our SF reprint this month is "Eminence," by Karl Schroeder. Jenny Rae Rappaport returns with "The Inheritance of Dust and Leather," a flash fantasy story that's a tale as old as time (or at least libraries). Kristina Ten's story of seals and bad marriages, "The Queen of the Earless Seals of Lake Baikal," will touch your heart, as will Martin Cahill's magical "Her Five Farewells." Our fantasy reprint is "The Sister City," by Cody Goodfellow. Our review teams have been busy reading great new books, and our author spotlight interviewer has put together fascinating questions for our writers. If you're one of our ebook readers, you'll enjoy an excerpt from DESERT CREATURES, a new novel by Kay Chronister.
John Joseph Adams
John Joseph Adams is the series editor of Best American Science Fiction & Fantasy. He is also the bestselling editor of many other anthologies, such as The Mad Scientist’s Guide to World Domination, Armored, Brave New Worlds, Wastelands, and The Living Dead. Recent books include The Apocalypse Triptych (consisting of The End is Nigh, The End is Now, and The End Has Come), and series editor for The Best American Fantasy and Science Fiction. John is a two-time winner of the Hugo Award and is a six-time World Fantasy Award finalist. John is also the editor and publisher of the digital magazines Lightspeed and Nightmare, and is a producer for WIRED’s The Geek’s Guide to the Galaxy podcast.
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Lightspeed Magazine, Issue 148 (September 2022) - John Joseph Adams
TABLE OF CONTENTS
Issue 148, September 2022
FROM THE EDITOR
Editorial: September 2022
SCIENCE FICTION
The CRISPR Cookbook: A Guide to Biohacking Your Own Abortion in a Post-Roe World
MKRNYILGLD
Civilian Assumptions
Dominique Dickey
The Application of Strawberry Lip Gloss in a Low-Gravity Environment
R J Theodore
Eminence
Karl Schroeder
FANTASY
The Inheritance of Dust and Leather
Jenny Rae Rappaport
The Queen of the Earless Seals of Lake Baikal
Kristina Ten
The Sister City
Cody Goodfellow
Her Five Farewells
Martin Cahill
EXCERPTS
Desert Creatures
Kay Chronister
NONFICTION
Book Review: She and Her Cat, by Makoto Shinkai, translated by Naruki Nagakawa
Aigner Loren Wilson
Book Review: Africa Risen, edited by Thomas, Ekpeki, & Knight
Arley Sorg
Book Review: Other Terrors, edited by Liaguno & Mason
Chris Kluwe
AUTHOR SPOTLIGHTS
Kristina Ten
RJ Theodore
Martin Cahill
MISCELLANY
Coming Attractions
Stay Connected
Subscriptions and Ebooks
Support Us on Patreon, or How to Become a Dragonrider or Space Wizard
About the Lightspeed Team
Also Edited by John Joseph Adams
© 2022 Lightspeed Magazine
Cover by Grandeduc / Adobe Stock Image
www.lightspeedmagazine.com
Published by Adamant Press
From_the_EditorEditorial: September 2022
John Joseph Adams | 240 words
Welcome to Lightspeed’s 148th issue!
Our first SF story of the month might be one of our most timely. From writer MKRNYILGLD comes The CRISPR Cookbook: A guide to biohacking your own abortion in a post-Roe world,
and the title does a great job describing it. Be sure not to miss it. Our flash SF this month, Civilian Assumptions,
comes from Dominique Dickey, while our second piece of full-length SF is a wildly delightful story of bounty hunters, space travel, and strawberry lip gloss. Yes: lip gloss. Please enjoy R J Theodore’s The Application of Strawberry Lip Gloss in a Low-Gravity Environment.
Our SF reprint this month is Eminence,
by Karl Schroeder.
Jenny Rae Rappaport returns with The Inheritance of Dust and Leather,
a flash fantasy story that’s a tale as old as time (or at least libraries). Kristina Ten’s story of seals and bad marriages, The Queen of the Earless Seals of Lake Baikal,
will touch your heart, as will Martin Cahill’s magical Her Five Farewells.
Our fantasy reprint is The Sister City,
by Cody Goodfellow.
Our review teams have been busy reading great new books, and our author spotlight interviewer has put together fascinating questions for our writers. If you’re one of our ebook readers, you’ll enjoy an excerpt from Desert Creatures, a new novel by Kay Chronister.
It’s another terrific month, so thanks for reading!
ABOUT THE AUTHOR
John Joseph Adams is the series editor of Best American Science Fiction and Fantasy and is the bestselling editor of more than thirty anthologies, including Wastelands and The Living Dead. Recent books include A People’s Future of the United States, Wastelands: The New Apocalypse, and the three volumes of The Dystopia Triptych. Called the reigning king of the anthology world
by Barnes & Noble, John is a two-time winner of the Hugo Award (for which he has been a finalist twelve times) and an eight-time World Fantasy Award finalist. John is also the editor and publisher of Lightspeed and is the publisher of its sister-magazines, Fantasy and Nightmare. For five years, he ran the John Joseph Adams Books novel imprint for Houghton Mifflin Harcourt. Find him online at johnjosephadams.com and @johnjosephadams.
The CRISPR Cookbook: A Guide to Biohacking Your Own Abortion in a Post-Roe World
MKRNYILGLD | 2380 words
If you’re reading this—on some godforsaken imageboard, or dog-eared book page, or in encrypted base pairs sequenced off 3D-printed oligos—you’re probably grappling with a pretty tough decision right now.
Breathe.
I’m not judging you. I know how it goes. You tried your best but nothing’s infallible, or you slipped up one night, or he just straight-up went, your biological clock’s ticking, and hacked your birth control, knowing once it happens you won’t have a choice. The second his sperm enters your egg, he’s done, back to his star-studded career cranking out Science and Cell papers, and you’re stuck at home—with everything from your calories to your screen time dictated to you by Big Brother—hoping your research project will still be waiting for you after the baby pops out.
Listen: in the twenty-five years since the Supreme Court’s reversal of Roe, we might’ve had our rights chipped away, little by little—but we’ve gained so much more. There’s a law of equivalent exchange that absolutely cannot be fucked with. The more protests get crushed on the streets, the more bubbles up underneath. And what’s under America is a bioreactor. A rising in every single one of the trillions of cells that make up our bodies, our chromosomes, in every single one of our tens of thousands of genes. They said you don’t have a choice, but, by reading this, you’re making one.
You’re taking back your DNA.
WHAT YOU’LL NEED
1. Pipettes. Own ten assault rifles and the cops won’t blink twice, but let the DHB catch you with these you’ll wind up in a re-ed camp faster than you can say homemade vole-pox.
Luckily, plenty of these puppies got dumped into circulation before the Homeland Biosecurity Act of 2030. Scour your local community groups for a helix motif, woven into baskets or crocheted into scarves. Tell the father, sweetly, that you’re going to pick up some organics for dinner. If your pregnancy officer makes a fuss, tell him you’ll bike over, the fresh air will be good for the baby.
You’re looking for a certain kind of stall, one that’s cropped up at farmers’ markets all across the nation. Their heirloom tomatoes will be slightly out of season, but still firework-bright. Their ears of corn will be popping with kernels of color you’ve never seen anywhere else. And perhaps the cat sitting in the shade will have a bit of a bioluminescent glow. Strike up a conversation with the vendor, say something like, Now that I’ve got months and months stuck at home, I’ve been thinking of experimenting with my garden too. And then you’ll be in.
2. Chemically competent cells, ideally RecA negative, at least six vials. The co-op you’ve made contact with will set you up with their microorganism dealer. Don’t fall for the sketchy types that’ll try to peddle you a virus they claim will solve your problem
directly. You don’t want to become patient zero for the latest MERS-Marburg superspreader cocktail. You might think you’re fighting back. But you’ll just end up becoming a carrier for someone else.
3. The plastics. AKA the disposables you need for everything you do in the lab: the pipette tips, the plates, the columns for DNA purification. The co-op will have some kind of 3D printer you can negotiate time on. It shouldn’t be difficult. These groups always need extra pairs of hands. You might find yourself caring for someone else’s cells or setting up PCR reactions or doing any other kind of grunt lab work. There’s no hierarchy here. Just a one-to-one exchange, hours for hours so everyone can be home on time to be the loving spouse. You might be helping someone produce insulin, or chemotherapy drugs, or hormones. No one asks so no one can be forced to talk, but in the case of a DHB bust you’re all getting shipped off to a re-ed camp, anyway. Who says the spirit of scientific collaboration is dead?
4. Transfection reagent. This is the stuff you’ll need to actually get your construct into your cells. Essentially positively-charged lipid particles that will help carry your payload through your cell membranes. Luckily, this shit is absolutely everywhere now that all our vaccines are nucleic acid-based. Find a sympathetic pharmacist and pay her under the table for some castoffs. Crack the bottles open. You’re going to need a few milliliters.
5. A CRISPR vector. I’m not talking about the first generation, straight-laced, Nobel-winning CRISPR/Cas9—or the off-the-walls, hyperactive, second generation Cas15-11. You need a third generation construct, the kind the DHB will break down your door in the middle of the night for if they catch wind of it. I’m talking synCas-X. It’s integral for all kinds of biological research now, but the feds slapped a Class Five Teratogen label on it, making it illegal for anyone at risk of getting pregnant to touch. Luckily, no one in the co-op’s going to tell you what you can and can’t grow.
By now you should’ve gained enough trust to get some time on the DNA printer (a pre-2030 model, or one that’s jailbroken to get around the lentiviral sequence filters). You’ll synthesize it block by block.
Meanwhile, you’re bringing home plenty of vegetables, making ratatouille, marinara sauce, healthy fresh meals night after night. Harvesting crops, you’ll tell your pregnancy officer when he asks about the farm you’re spending so much time on. Potluck clubs with the girls. You’ll make him fried squash blossoms, better than any of the crap he gets in the mess hall, and he won’t suspect a thing. And when you finally get those microliters of vector, you’ll hold it up to the light. You’ll marvel that synCas-X can look like nothing at all.
THE PRINCIPLE
It’s actually very simple. What you’re going to do is hack your immune system. Think about it. What’s the immune system’s job? It detects foreign, or non-self, cells and destroys them. But it doesn’t always succeed. One of immunology’s biggest mysteries was how an embryo can develop without triggering the mother’s immune system. I say was, because in 2037, scientists finally figured it out. Like many biologists who solved something big, they were working in another field entirely, without even realizing the significance of their findings.
Cancer is another blind spot of the immune system. In part because tumor cells share so much DNA with their host—they are their host, plus a scattering of cancerous mutations. Scientists had reasoned for decades that they could tweak the immune system to go after those little variations. They tried combinations on combinations of gene deletions, tweaks, duplications, charged patients millions for the hope of personalized medicines. But they could never get it to work. Even their souped-up immune cells weren’t sensitive enough. Models predicted that 40% of a tumor’s genome would have to differ from the host’s as much as another person’s does in order for the treatment to work.
Do you see where I’m going with this? What contains even more non-self DNA than a tumor? What gets 50% of its DNA from another individual entirely? An individual who’s taken to coming home and wrapping his arms around you, hands on your stomach, after a hard day’s work—whispering in your ear about how he knows how hard it’s been for you, but he just has to get this faculty position, then he’ll be there for you and the baby 100%, he’ll make it up to you, he’s asking for a small lab for you to run, attached to his own. And have you started thinking of names for your son?
THE PROCEDURE
1. Design guide RNAs to knock out the following immune checkpoint genes: PDCD1, CD274, CTLA4, TIGIT, LAG3, and their little friend FAM594B. You know the drill: Put them as early in the first exon after the start codon as possible, with BbsI restriction sites at the ends. Print the oligos out, anneal them in a water bath brought to boil and cooled, and clone them into your CRISPR vector. It’s normal to have failed reactions, especially on hot nights. Does it remind you of your first research internship, all the flies buzzing around? Wipe your tears and push through.
2. Transform your vectors into your chemically competent bacteria. Grow up two liters under selection and purify using a standard maxi-prep kit. Tell your pregnancy officer you’re babysitting late for a friend, learning everything you can in preparation. You have to move quick. You don’t want this to take more than a few weeks.
3. Draw ten milliliters of your blood. Pipette five milliliters on top of five milliliters of your favorite density gradient. Spin it down at 500g for one hour at room temperature. You want the peripheral blood mononuclear cell layer. It’s the cloudy layer right under the layer of plasma. If you’re having trouble pipetting it out, ask for help. The co-op gets it—the single moms in kerchiefs, the teen runaways showing their hair loss proud. Isolating T-cells is a standard protocol for homebrew cancer therapies.
4. Transfer the PBMCs to a 3D-printed 10 cm plate. Incubate for one hour. This will separate the monocytes, which should stick to the plate bottom, from the lymphocytes, which include your T-cells (along with B-cells, which are a pain to separate, so it’s fine if they come along too). If nothing or everything is sticking, something’s probably wrong with your 3D printer filament. Trash the plate and try again.
5. The next day, your lymphocytes will be ready for transfection. Mix the CRISPR construct purified from your competent cells with your vaccine lipid particles. Incubate for half an hour and pipette on top of your lymphocytes. Incubate for forty-eight hours and replace with fresh media. Let them grow for four more days. This is the easy part.
6. Harvest your lymphocytes and deliver them back into your bloodstream via intravenous infusion. Now’s the time to check out your co-op’s book or knitting club. It’s what it sounds like, except everyone hooks up their homebrew chemo or antiviral IV bags before settling in for the afternoon. You’ll feel like shit.
7. Get yourself sick. Now’s the time to grab yourself a weak version of