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Multi-monoubiquitination controls VASP-mediated actin dynamics

Multi-monoubiquitination controls VASP-mediated actin dynamics

FromPaperPlayer biorxiv cell biology


Multi-monoubiquitination controls VASP-mediated actin dynamics

FromPaperPlayer biorxiv cell biology

ratings:
Length:
20 minutes
Released:
Jul 17, 2023
Format:
Podcast episode

Description

Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.07.16.549237v1?rss=1

Authors: McCormick, L. E., Suarez, C., Herring, L. E., Cannon, K. S., Kovar, D. R., Brown, N. G., Gupton, S. L.

Abstract:
The actin cytoskeleton performs multiple cellular functions, and as such, actin polymerization must be tightly regulated. We previously demonstrated that reversible, non-degradative ubiquitination regulates the function of the actin polymerase VASP in developing neurons. However, the underlying mechanism of how ubiquitination impacts VASP activity was unknown. Here we show that mimicking multi-monoubiquitination of VASP at K240 and K286 negatively regulates VASP interactions with actin. Using in vitro biochemical assays, we demonstrate the reduced ability of multi-monoubiquitinated VASP to bind, bundle, and elongate actin filaments. However, multi-monoubiquitinated VASP maintained the ability to bind and protect barbed ends from capping protein. Lastly, we demonstrate the introduction of recombinant multi-monoubiquitinated VASP protein altered cell spreading morphology. Collectively, these results suggest a mechanism in which ubiquitination controls VASP-mediated actin dynamics.

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Podcast created by Paper Player, LLC
Released:
Jul 17, 2023
Format:
Podcast episode

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