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CLASPs stabilize the intermediate state between microtubule growth and catastrophe
CLASPs stabilize the intermediate state between microtubule growth and catastrophe
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Length:
20 minutes
Released:
Dec 4, 2022
Format:
Podcast episode
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Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.12.03.518990v1?rss=1
Authors: Lawrence, E. J., Chatterjee, S., Zanic, M.
Abstract:
CLASPs regulate microtubules in many fundamental cellular processes. CLASPs stabilize dynamic microtubules by suppressing catastrophe and promoting rescue, the switch-like transitions between microtubule growth and shrinkage. However, the molecular mechanisms underlying CLASPs activity are not understood. Here, we investigate the effects of CLASPs on distinct microtubule substrates in the absence of tubulin to gain insight into how CLASPs regulate microtubule dynamics. Surprisingly, we find that human CLASP1 depolymerizes stable microtubules in the presence of GTP, but not in the absence of nucleotide. Conversely, CLASP1 stabilizes dynamic microtubules upon tubulin dilution in the presence of GTP. Our results demonstrate that CLASP1 drives microtubule substrates with different inherent stabilities into the same slowly-depolymerizing state in the absence of tubulin in a nucleotide-dependent manner. We interpret this state as the pre-catastrophe intermediate state between microtubule growth and shrinkage. Thus, we conclude that CLASPs stabilize the intermediate state between microtubule growth and shrinkage to suppress microtubule catastrophe and promote rescue.
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Podcast created by Paper Player, LLC
http://biorxiv.org/cgi/content/short/2022.12.03.518990v1?rss=1
Authors: Lawrence, E. J., Chatterjee, S., Zanic, M.
Abstract:
CLASPs regulate microtubules in many fundamental cellular processes. CLASPs stabilize dynamic microtubules by suppressing catastrophe and promoting rescue, the switch-like transitions between microtubule growth and shrinkage. However, the molecular mechanisms underlying CLASPs activity are not understood. Here, we investigate the effects of CLASPs on distinct microtubule substrates in the absence of tubulin to gain insight into how CLASPs regulate microtubule dynamics. Surprisingly, we find that human CLASP1 depolymerizes stable microtubules in the presence of GTP, but not in the absence of nucleotide. Conversely, CLASP1 stabilizes dynamic microtubules upon tubulin dilution in the presence of GTP. Our results demonstrate that CLASP1 drives microtubule substrates with different inherent stabilities into the same slowly-depolymerizing state in the absence of tubulin in a nucleotide-dependent manner. We interpret this state as the pre-catastrophe intermediate state between microtubule growth and shrinkage. Thus, we conclude that CLASPs stabilize the intermediate state between microtubule growth and shrinkage to suppress microtubule catastrophe and promote rescue.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Dec 4, 2022
Format:
Podcast episode
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