Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.04.13.536018v1?rss=1

Authors: Stalder, D., Yakunin, I., Gershlick, D. C.

Abstract:
ACBD3 is a protein known to localise to the Golgi apparatus, and recruits various proteins to the Golgi, including PI4KIIIbeta, but the mechanism of its recruitment has remained unclear. This study demonstrates there are two mechanisms for ACBD3 recruitment to the Golgi. First, we identified that an MWT374-376 motif in the unique region upstream of the GOLD domain in ACBD3 is essential for Golgi localisation. Second, we use unbiased proteomics to demonstrate that ACBD3 interacts with SCFD1, a Sec1/Munc-18 (SM) protein, and a SNARE protein, SEC22B. CRISPR-KO of SCFD1 causes ACBD3 to become cytosolic. We also found that ACBD3 is redundantly recruited to the Golgi apparatus by two golgins, golgin-45 and giantin, which bind to ACBD3 through interaction with the MWT374-376 motif. Taken together, our results demonstrate that ACBD3 is recruited to the Golgi in a two-step sequential process, with the SCFD1-mediated interaction occurring upstream of the interaction with the golgins.

Copy rights belong to original authors. Visit the link for more info

Podcast created by Paper Player, LLC