Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.02.15.528649v1?rss=1

Authors: Wang, B., Liang, Z., Tan, T., Zhang, M., Jiang, Y., Shang, Y., Gao, X., Song, S., Wang, R., Chen, H., Liu, J., Li, J., Ren, Y., Liu, P.

Abstract:
The primary cilium plays important roles in regulating cell differentiation, signal transduction, and tissue organization. Dysfunction of the primary cilium can lead to ciliopathies and cancer. The formation and organization of the primary cilium are highly associated with cell polarity proteins, such as the apical polarity protein CRB3. However, the molecular mechanisms by which CRB3 regulates ciliogenesis and CRB3 location remain unknown. Here, we show that CRB3, as a navigator, regulates vesicle trafficking in {gamma}-TuRC assembly during ciliogenesis and cilium-related Hh and Wnt signaling pathways in tumorigenesis. Crb3 knockout mice display severe defects of the primary cilium in the mammary ductal lumen and renal tubule. CRB3 is essential for lumen formation and ciliary assembly in the mammary epithelium. We demonstrate that CRB3 localizes to the basal body and that CRB3 trafficking is mediated by Rab11-positive endosomes. Significantly, CRB3 directly interacts with Rab11 to navigate GCP6/Rab11 trafficking vesicles to CEP290, resulting in intact {gamma}-TuRC assembly. In addition, CRB3-depleted cells cannot respond to the activation of the Hh signaling pathway, while CRB3 regulates the Wnt signaling pathway. Therefore, our studies reveal the molecular mechanisms by which CRB3 recognizes Rab11-positive endosomes to navigate apical vesicle trafficking in effective ciliogenesis, maintaining cellular homeostasis and tumorigenesis.

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