Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.12.10.519870v1?rss=1

Authors: Fung, H. K., Hayashi, Y., Salo, V. T., Babenko, A., Zagoriy, I., Brunner, A., Ellenberg, J., Mueller, C. W., Cuylen-Haering, S., Mahamid, J.

Abstract:
Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.

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