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Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM)
Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM)
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Length:
20 minutes
Released:
Nov 15, 2022
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.11.14.516383v1?rss=1
Authors: Louvel, V., Haase, R., Mercey, O., Laporte, M. H., Soldati-Favre, D., Hamel, V., Guichard, P.
Abstract:
Expansion microscopy is an approach of super-resolution fluorescence microscopy that does not yet achieve the precision of nanoscopy techniques such as single-molecule light microscopy (SMLM). Here, we developed an iterative ultrastructure expansion microscopy approach (iU-ExM), which now matches the SMLM resolution as demonstrated using standard references such as the nuclear pores. Applicable to both cells and tissues, iU-ExM allows a broad research community to access high precision super-resolution microscopy.
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Podcast created by Paper Player, LLC
http://biorxiv.org/cgi/content/short/2022.11.14.516383v1?rss=1
Authors: Louvel, V., Haase, R., Mercey, O., Laporte, M. H., Soldati-Favre, D., Hamel, V., Guichard, P.
Abstract:
Expansion microscopy is an approach of super-resolution fluorescence microscopy that does not yet achieve the precision of nanoscopy techniques such as single-molecule light microscopy (SMLM). Here, we developed an iterative ultrastructure expansion microscopy approach (iU-ExM), which now matches the SMLM resolution as demonstrated using standard references such as the nuclear pores. Applicable to both cells and tissues, iU-ExM allows a broad research community to access high precision super-resolution microscopy.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Nov 15, 2022
Format:
Podcast episode
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