LABORATORY MANUAL FOR A MINI PROJECT: MSCB 1113 BIOCHEMISTRY & MICROBIAL PHYSIOLOGY

By Fahrul Huyop

The lab manual for a mini-project is intended for use by MSc level students at Universiti Teknologi Malaysia. The manual is designed to help students plan and execute a mini-project, which is a required component of their academic curriculum in their final year. The lab manual will include four important experiments covering microbial growth on selected substrates, followed by DNA analysis and protein analysis by SDS-electrophoresis of bacterial growth in the selected medium. This book will serve as a basic guide for students.

• Language: English
• Publisher: Lulu.com
• Release date: May 8, 2023
• ISBN: 9781312692886

Book preview

Safety Rules in Laboratory

The following procedures should be followed at all times to ensure safety in the laboratory.

1. Please be prepared before attending the practical class. By being familiar with the exercise, you can minimize the chances of unnecessary accident. In addition, it will also allow you to use your time effectively in the laboratory, thus completing your experiment on time.

2. EATING, DRINKING OR SMOKING IS NOT ALLOWED IN THE LABORATORY. The University is a strictly non-smoking prohibited area.

3. Lab coat and goggle SHOULD be worn at all times while working in the laboratory to protect clothes, skin, and eye from chemicals and stains.

4. Only those materials pertinent to your laboratory work, such as laboratory manual, lab notebook and other lab materials should be brought in the laboratory workplace. All other items such as coats, books and bags should be kept in the cabinet.

5. In case of any emergencies or accidents, please report it immediately to the supervisor or lab assistant for immediate action.

General Introduction

During the lab session, students will have the opportunity to develop various important skills. They will learn how to design and plan experiments, and become proficient in