Atlas of Zebrafish Development

By Robert Bryson-Richardson, Silke Berger and Peter Currie

Zebrafish are widely considered an excellent model system for vertebrate development. The embryo is transparent, thereby enabling visualization and use of labelling and transgenic approaches. Moreover, because of the ease of inducing new mutations in zebrafish and similarity with the human genome, this organism may be used effectively for disease studies. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from Alzheimer's and cancer to kidney failure and congenital heart disease. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood.

The authors employ the technique of optical projection tomography (OPT), and offer a series of sections in multiple planes from each sample. The contents are organized by developmental stages, with over 200 images that contain annotations describing anatomical structures relevant to development. In addition, chapters feature explanatory text that highlights major developments in the zebrafish during each stage.

• Provides the first comprehensive anatomical resource that covers all regions of zebrafish anatomy from the larval period to adulthood
• The over 200 images include explanatory notes
• Each chapter contains a concise description of key anatomical features that factor in zebrafish development
• Despite many years of use as a model system, until now there has never been a guide to zebrafish at the larval stage
• The book's website contains a database of over 10k sections from different regions as well as 3D images that are interactive

• Language: English
• Publisher: Academic Press
• Release date: Aug 8, 2011
• ISBN: 9780080920610

Book preview

Table of Contents

Cover image

Front-matter

Copyright

Chapter 1. Introduction

Chapter 2. Methodology and Interpretation of the Atlas

Chapter 3. FishNet

Chapter 4. 24 Hours

Chapter 5. 48 Hours

Chapter 6. 72 Hours

Chapter 7. 96 Hours

Chapter 8. 120 Hours

Chapter 9. 6 MM

Chapter 10. 9 MM

Chapter 11. 14 MM

Chapter 12. 16 MM

References

Index

Front-matter

Atlas of Zebrafish Development

Atlas of Zebrafish Development

R

obert

B

ryson

-R

ichardson

, School of Biological Sciences, Monash University

S

ilke

B

erger

, Australian Regenerative Medicine Institute, Monash University

P

eter

C

urrie

, Australian Regenerative Medicine Institute, Monash University

Academic Press is an imprint of Elsevier

Copyright

Academic Press is an imprint of Elsevier

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First edition 2012

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ISBN : 978-0-12-374016-8

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Chapter 1. Introduction

Summary

The Atlas of Zebrafish Development provides a gross anatomical reference for the zebrafish, Danio rerio. Utilising the technique of optical projection tomography we generated three-dimensional models of zebrafish spanning the complete development of the fish from 24 hours post fertilization until adulthood. Virtual sections have been generated from these three-dimensional models at representative stages and annotated to provide the first atlas of zebrafish development. In addition to the annotated images of zebrafish development we provide a description of the need for the atlas, the technique used to generate it, and information on the use of the accompanying website, FishNet, which complements the atlas.

Keywords

Model organism, resource, anatomy, zebrafish, Danio rerio

The Need for an Atlas of Zebrafish Development

Over the past two decades zebrafish have been established as a genetically tractable model system to investigate many different aspects of vertebrate development. The strengths of this model system stem from a unique combination of embryological manipulability and the optical clarity of the early embryo and larvae, which allows, by simple optical inspection, the visualization of cell biological events within an in vivo context. This is further enhanced by the ability