DNA Engineered Noble Metal Nanoparticles: Fundamentals and State-of-the-Art of Nanobiotechnology
By Ignac Capek
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About this ebook
There is a growing interest in the use of nanoparticles modified with DNAs, viruses, peptides and proteins for the rational design of nanostructured functional materials and their use in biosensor applications. The challenge is to control the organization of biomolecules on nanoparticles while retaining their biological activity as potential chemical and gene therapeutics. These noble metal nanoparticles/biomolecules conjugates have specific properties and therefore they are attractive materials for nanotechnology in biochemistry and medicine.
In this book, the author review work performed dealing with the DNA structure and functionalities, interactions between DNA, noble metal nanoparticles, surface active agents, solvents and other additives. Particular attention is given to how the DNA’s chain length and the DNA conformation affect the interaction and structure of the nanoconjugates and nanostructures that are formed. Also discussed are the recent advances in the preparation, characterization, and applications of noble metal nanoparticles that are conjugated with DNA aptamers and oligomers. The advantages and disadvantages of functionalized nanoparticles through various detection modes are highlighted, including colorimetry, fluorescence, electrochemistry, SPR, and, mass spectrometry for the detection of small molecules and biomolecules. The functionalized noble metal nanoparticles are selective and sensitive for the analytes, showing their great potential in biosensing. Furthermore, this book reviews recent progress in the area of DNA-noble metal nanoparticles based artificial nanostructures, that is, the preparation, collective properties, and applications of various DNA-based nanostructures are also described.
Ignac Capek
Professor Ignác Capek studied at Waterloo University, Ontario, Canada, Stuttgart University, Germany (supported by Humboldt Fellowship), Kagoshima University, Japan (supported by the Japan Society for the promotion of Science), Maramara Res. Center, Turkey, San Sebastian University, Spain, Mulhouse University, France, and National Taiwan University, Taipei, Taiwan. His research topics encompass: Preparation of conventional and nonconventional polymer and polymer/metal dispersions and metal fluids. Modification, functionalization and decoration of polymer and metal nanoparticles, dendrimers, CNTs, lyposomes, etc. by biomolecules (aptamers, oligomers, peptides, antibodies, antigens, etc). Development of biosensors on the base of polymer and metal nanoparticles, DNA aptamers, oligopeptides, oligosaccharides, enzymes, etc. Professor I. Capek is author and co-author of 7 scientific books, 25 chapters, ca. 200 scientific papers and more than 25 patents. Literární Fond granted professor I. Capek several awards in the scientific literature in 1996, 2006, 2016 and 2018 for the scientific books (Radical Polymerization in Disperse Systems), Elsevier granted him for a paper published in Adv. Colloid Interface Sci. the Most Cited Paper 2004 – 2007 Award. International agency ARRA included research team leading by prof. I. Capek into the category of extraordinary teams. He is the member of several well-known scientific societies such as: Alexander von Humboldt (Germany), Japan Society for the Promotion of Science (Japan), etc.
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