Science behind Non-specific Science: (For Molecular Biologist & Biotechnologist)

By Vikash Bhardwaj

The cover page of my book shows factsheets data available on the W.H.O. website. We can see, millions of people have died and many millions more will die in the coming future due to various diseases. Throughout the world, trillions of dollars are being invested to find solutions to various diseases and many more trillions of dollars will be invested in the coming future. All over the world scientists do perform experiments using well established protocols with or without minor modification as per their experimental approach. In this book, I have discussed a possible hypothesis behind ‘Non-specific results’ obtained by four techniques (Southern blotting, Northern blotting, Microarray, siRNA technology) which are widely used in molecular biology research. I have also tried to give a better hypothetical solution which can minimize experimental errors. I will introduce you to a broader definition of “Complementary” in DNA structure (which has never been discussed in standard biology books), a novel PD-PCR technology developed by me and presence of novel mirror repeat sequences within most of human genes. Hopefully knowledge within this book will be helpful in developing various molecular biology techniques which will not show any experimental error. Doing scientific experiments without any error will only solve the sufferings of mankind. After reading my book, feel to answer my only question, “Over a period of time, have we collected valid scientific data to be used by coming generation of young scientist in molecular biology research?”

• Language: English
• Publisher: Notion Press
• Release date: Sep 5, 2014
• ISBN: 9789384049430

Book preview

Glossary

Chapter 1

Complementary: What It Means Broadly?

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Introduction

In early history of modern science, proteins were considered most key target molecules to study how cell function. Much of research was focused on characterizing various protein components found in a cell. In 1868/69 a young Swiss physician and scientist Freidrich Miescher working in the laboratory of distinguished biochemist Felix Hoppe-Seyler (discoverer of hemoglobin) at University of Tubingen in Germany, planned to isolate and characterize protein components of leukocytes (white blood cells). From a local surgical clinic, Miescher used to take fresh pus-coated patient’s bandages. Once he received the bandages, he filtered out leukocytes, and used to extract and identify various biological components within white blood cells nuclei using various acidic and alcoholic solutions. He was surprised when he came across a novel substance from cell nuclei that had chemical properties unlike any protein. He found that new class of cellular substance which he has discovered consists of nitrogen, a much higher phosphorous content without sulphur and is resistant to proteolysis. Miescher coined it the term Nuclein. Although Miescher did most of his work in 1869, his paper on Nuclein wasn’t published until 1871. Nuclein was such a unique molecule that Hoppe-Seyler was skeptical about these findings. After a year, Hoppe-Seyler was convinced with Miescher’s results and published those in a journal edited by himself. So Mischer is being credited person who first time isolated nuclei and discovered DNA, a biological molecule which is now well considered as a key to some of the greatest mysteries of life. Later, Miescher also proposed many ideas about biological functions of this molecule. As stated by R. Dahm, Miescher was a brilliant scientist well on his way to making one of the most fundamental discoveries in the history of science, who ultimately fell short of his potential because he clung to established theories and failed to follow through with the interpretation of his findings in a new light