The Nature of Animal Light

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Title: The Nature of Animal Light

Author: E. Newton Harvey

Release Date: November 26, 2010 [EBook #34450]

Language: English

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PREFACE

CONTENTS

BIBLIOGRAPHY

INDEX

Monographs On Experimental Biology

EDITED BY

JACQUES LOEB, Rockefeller Institute

T. H. MORGAN, Columbia University

W. J. V. OSTERHOUT, Harvard University

THE NATURE OF ANIMAL LIGHT

BY

E. NEWTON HARVEY, Ph.D.

MONOGRAPHS ON EXPERIMENTAL BIOLOGY

PUBLISHED

FORCED MOVEMENTS, TROPISMS, AND ANIMAL CONDUCT

By JACQUES LOEB, Rockefeller Institute

THE ELEMENTARY NERVOUS SYSTEM

By G. H. PARKER, Harvard University

THE PHYSICAL BASIS OF HEREDITY

By T. H. MORGAN, Columbia University

INBREEDING AND OUTBREEDING: THEIR GENETIC AND SOCIOLOGICAL SIGNIFICANCE

By E. M. EAST and D. F. JONES, Bussey Institution, Harvard University

THE NATURE OF ANIMAL LIGHT

By E. N. HARVEY, Princeton University

IN PREPARATION

PURE LINE INHERITANCE

By H. S. JENNINGS, Johns Hopkins University

THE EXPERIMENTAL MODIFICATION OF THE PROCESS OF INHERITANCE

By R. PEARL, Johns Hopkins University

LOCALIZATION OF MORPHOGENETIC SUBSTANCES IN THE EGG

By E. G. CONKLIN, Princeton University

TISSUE CULTURE

By R. G. HARRISON, Yale University

PERMEABILITY AND ELECTRICAL CONDUCTIVITY OF LIVING TISSUE

By W. J. V. OSTERHOUT, Harvard University

THE EQUILIBRIUM BETWEEN ACIDS AND BASES IN ORGANISM AND ENVIRONMENT

By L. J. HENDERSON, Harvard University

CHEMICAL BASIS OF GROWTH

By T. B. ROBERTSON, University of Toronto

COÖRDINATION IN LOCOMOTION

By A. R. MOORE, Rutgers College

OTHERS WILL FOLLOW

Monographs on Experimental Biology

THE NATURE OF ANIMAL LIGHT

BY

E. NEWTON HARVEY, Ph.D.

PROFESSOR OF PHYSIOLOGY, PRINCETON UNIVERSITY

PHILADELPHIA AND LONDON J. B. LIPPINCOTT COMPANY

COPYRIGHT, 1920. BY J. B. LIPPINCOTT COMPANY

Electrotyped and Printed by J. B. Lippincott Company.

The Washington Square Press, Philadelphia, U. S. A.

EDITORS' ANNOUNCEMENT

The rapid increase of specialization makes it impossible for one author to cover satisfactorily the whole field of modern Biology. This situation, which exists in all the sciences, has induced English authors to issue series of monographs in Biochemistry, Physiology, and Physics. A number of American biologists have decided to provide the same opportunity for the study of Experimental Biology.

Biology, which not long ago was purely descriptive and speculative, has begun to adopt the methods of the exact sciences, recognizing that for permanent progress not only experiments are required but quantitative experiments. It will be the purpose of this series of monographs to emphasize and further as much as possible this development of Biology.

Experimental Biology and General Physiology are one and the same science, in method as well as content, since both aim at explaining life from the physico-chemical constitution of living matter. The series of monographs on Experimental Biology will therefore include the field of traditional General Physiology.

Jacques Loeb,

T. H. Morgan,

W. J. V. Osterhout.

PREFACE

Bioluminescence, the production of light by animals and plants, has always excited the admiration of the layman and the wonder of the scientist. It is not surprising that an enormous literature dealing with the subject has grown up. A large part of this literature, however, is made up merely of reports that a certain animal is luminous, or records of especially brilliant phosphorescence of the sea. Among those who have inquired somewhat more carefully into the nature and causes of light production may be mentioned the names of Beijerinck, R. Boyle, Dahlgren, Dubois, Ehrenberg, Krukenberg, Mangold, McDermott, Molisch, Panceri, Pflüger, Phipson, Quatrefages, Spallanzani, and Trojan. Several of these men have written comprehensive monographs on the subject.

It is not the purpose of this book to deal with every phase of bioluminescence. Volumes could be written on the evolutionary side of the problem and the structure and uses of luminous organs. These questions can only be touched upon. Neither is it my purpose to discuss the ultimate cause of the light, whether due to vibration of electrons or to other causes. That problem must be left to the physicist, although it is highly probable that a study of animal light will give important information regarding the nature of light in general, and no theory of light can be adequate which fails to take into account the extraordinary powers of luminous animals.

We shall be concerned largely with the physical characteristics of animal light and the chemical processes underlying its production. Great advances have been made since the first early guesses that the light was due to phosphorus and was a kind of oxidation. Although the problem cannot be considered as solved, it has been placed on a sound physico-chemical basis. Some material is oxidized. Exactly what this material is and why light accompanies its oxidation are the two more fundamental problems in the field of Bioluminescence. How far and with what success we have progressed toward a solution of these problems may be seen from a perusal of the following pages.

It gives me pleasure to acknowledge the kindness of Dr. W. E. Forsythe of the Nela Institute, Cleveland, Ohio, in reading and criticizing the manuscript of Chapter III, and of Professor Lyman of Harvard University for a similar review of Chapter II. I am also deeply indebted to my wife for reading the proof and to Dr. Jacques Loeb and Prof. W. J. V. Osterhout for many suggestions throughout the book. My thanks are also due to Prof. C. Ishikawa of the Agricultural College, Imperial University of Tokio, Japan, for his generous assistance in providing Cypridina material. Finally I wish to acknowledge the support of the Carnegie Institution of Washington, through its director of Marine Biology, Dr. Alfred G. Mayor. Without this support much of the work described in this book could not have been accomplished.

E. N. H.

Princeton, N. J.,

October, 1919.

CONTENTS

THE NATURE OF ANIMAL LIGHT

CHAPTER I

LIGHT-PRODUCING ORGANISMS

The fact that animals can produce light must have been recognized from the earliest times in countries where fireflies and glowworms abound, but it is only since the perfection of the microscope that the phosphorescence of the sea, the light of damp wood and of dead fish and flesh has been proved to be due to living organisms. Aristotle mentions the light of dead fish and flesh and both Aristotle and Pliny that of damp wood. Robert Boyle in 1667 made many experiments to show that the light from all three sources, as well as that of the glowworm, is dependent upon a plentiful supply of air and drew an interesting comparison between the light of shining wood and that of a glowing coal. Boyle had no means of finding out the true cause of the light and early views of its nature were indeed fantastic. Even as late as 1800 Hulme concludes from his experiments on phosphorescent fish that the light is a constituent principle of marine fishes and the first that escapes after the death of the fish. It was only in 1830 that Michaelis suspected the light of dead fish to be the result of some living thing and in 1854 Heller gave the name Sarcina noctiluca to the suspected organism. In 1875 Pflüger showed that nutrient media could be inoculated with small amounts of luminous fish and that these would increase in size, like bacterial colonies, and we now know that the light of all dead fish and flesh is due to luminous bacteria.

In the early part of the nineteenth century it was surmised that the light of damp wood was connected with fungus growth because of a similarity in smell. In 1854 Heller recognized minute strands, which he called Rhizomorpha noctiluca, as the actual source of the light. We now know that all phosphorescent wood is due to the mycelium of various kinds of fungi and that sometimes the fruiting body of the fungus also produces light.

The phosphorescence or burning of the sea, which is described by so many of the older explorers, is also due entirely to living organisms, both microscopic and macroscopic. The latter are mostly jelly-fish (medusæ) or comb jellies (Ctenophores) and give rise to the larger, more brilliant flashes of light often seen in the wake or about the sides of a steamer at night. The former are various species of dinoflagellates or cystoflagellates such as Noctiluca (just visible to the naked eye) which collect at the surface of the sea and often increase in such numbers that the water is colored by day (usually pink or red) and shines like a sheet of fire when disturbed at night. Although Noctiluca was recognized as a luminous animal in 1753 by Baker, the light of the sea was a mysterious phenomenon to the older observers. MacCartney, speaking before the Royal Society in 1810, outlines the various older theories as follows: Many writers have ascribed the light of the sea to other causes than luminous animals. Martin supposed it to be occasioned by putrefaction; Silberschlag believed it to be phosphoric; Prof. J. Mayer conjectured that the surface of the sea imbibed light, which it afterwards discharged. Bajon and Gentil thought the light of the sea was electric, because it was excited by friction.... I shall not trespass on the time of the Society to refute the above speculations; their authors have left them unsupported by either arguments or experiments, and they are inconsistent with all ascertained facts upon the subject. The remarkable property of emitting light during life is only met amongst animals of the four last classes of modern naturalists, viz., mollusca, insects, worms, and zoöphytes. MacCartney recognized the true cause of the light, although he had little idea of the vast number of marine forms which are luminous and omits entirely any reference to the fishes, many of which produce a light of their own when living, apart from any bacterial infection.

A survey of the animal kingdom discloses at least 36 orders containing one or more forms known to produce light and several more orders containing species whose luminosity is doubtful. In the plant kingdom there are two groups containing luminous forms. The distribution of luminous organisms is brought out in the accompanying classification of plants and animals. Those orders are printed in italics which contain species whose self-luminosity is fairly well established. It will be noted that further subdivisions into orders is not given in classes of animals which lack luminous forms.

TABLE 1

DISTRIBUTION OF LUMINOUS ORGANISMS IN PLANT AND ANIMAL KINGDOMS

Plant Kingdom

I. Thallophyta

Algæ

Cyanophyceæ (Blue-green Algæ)

Chlorophyceæ (Green Algæ)

Phæophyceæ (Brown Algæ)

Rhodophyceæ (Red Algæ)

Lichenes (Lichens, symbiotic growth of algæ and fungi)

Fungi

Myxomycetes (Slime moulds)

Schizomycetes (Bacteria)

Bacterium, Photobacterium, Bacillus, Pseudomonas, Micrococcus, Microspira, Vibrio.

Phycomycetes (moulds)

Ascomycetes (Sac fungi, yeasts, some moulds)

Basidiomycetes (Smuts, rusts, mushrooms)

Ustilaginæ (Smuts)

Uridineæ

Auriculariæ (Judas ears)

Tremellineæ (Jelly fungi)

Hymenomycetes (Mushrooms)

Agaricus, Armillaria, Pleurotus, Panus, Mycena, Omphalia, Locellina, Marasinium, Clitocybe, Corticium.

Gasteromycetes (Stinkhorns and puff-balls)

II. Bryophyta

Hepaticæ (Liverworts)

Musci (Mosses)

III. Pteridophyta

Equisetineæ (Horsetails)

Salviniæ (Salvinia, Marsilia, etc.)

Lycopodineæ (Club Mosses)

Filicineæ (Ferns)

IV. Spermatophyta

Gymnospermæ (Cycads, Ginkgo, Conifers)

Angiospermæ (Mono- and Dicotyledonous flowering plants).

Animal Kingdom

I. Protozoa. (One-celled animals)

Sarcodina

Rhizopoda

Heliozoa

Radiolaria

Thallassicola, Myxosphæra, Collosphæra, Collozoum, Sphærozoum.

Mastigophora

Flagellata

Choanoflagellata

Dinoflagellata

Ceratium, Peridinium, Prorocentrum, Pyrodinium, Gonyaulax, Blepharocysta, Amphidinium, Diplopsalis, Cochlodinium, Sphærodinium, Gymnodinium.

Cystoflagellata

Noctiluca, Pyrocystis, Leptodiscus, Craspedotella.

Sporozoa

Infusoria

II. Porifera (Sponges)

Calcarea

Hexactinellida

Desmospongiæ

III. Cœlenterata

Hydrozoa (Hydroids and Jelly-fish)

Leptomedusæ or Campanulariæ

Medusa form—Eutima, Phyalidium (Oceania).

Hydroid form—Aglaophenia, Campanularia, Sertularia, Plumularia, Cellularia, Valkeria, Obelia, Clytia.

Trachomedusæ

Geryonia, Lyriope, Aglaura

Narcomedusæ

Cunina

Anthomedusæ or Tubulariæ

Medusa form—Thaumantias, Tiara, Turris, Sarsia.

Hydroid form—?

Hydrocorallinæ

Siphonophora

Abyla, Praya, Diphyes, Eudoxia, Hippopodius.

Scyphozoa (Jelly-fish)

Stauromedusæ

Peromedusæ

Cubomedusæ

Carybdia

Discomedusæ

Pelagia, Aurelia, Chrysaora, Rhizostoma, Cyanæa, Dianea, Mesonema.

Actinozoa (Corals, Sea-fans, Sea-pens, Sea-anemones)

Actinaria

Madreporareia

Antipatharia

Alcyonaria

Alcyonium, Gorgonia, Isis, Mopsea

Pennatulacea

Pennatula, Pteroides, Veretillum, Cavernularia.

Funicularia, Renilla, Pavonaria, Stylobelemon, Umbellularia, Virgularia?

Ctenophora (Comb-jellies)

Cydippida

Pleurobranchia.

Lobata

Mnemiopsis, Bolinopsis, Leucothea (Eucharis).

Cestida

Cestus.

Beroida

Beroë.

IV. Platyhelminthes

Turbellaria (Flat-worms)

Trematodes (Parasitic flat-worms)

Cestodes (Tape-worms)

Nemertinea (Nemertines)

V. Nemathelminthes

Nematoda (Round worms)

Gordiacea (Hair worms)

Acanthocephala (Acanthocephalids)

Chætognatha (Sagitta)

VI. Trochelminthes

Rotifera (Wheel animalcules)

Gastrotricha (Chætonotus)

Kinorhyncha (Echinoderes)

VII. Molluscoidea

Bryozoa (Corallines)

Entoprocta

Ectoprocta

Membranipora, Scrupocellaria, Retepora?Flustra?

Brachiopoda (Lamp shells)

Phoronidea (Phoronis)

VIII. Annulata

Archiannelida (Primitive worms, including Dinophilus)

Chætopoda (True worms)

Polychæta

Chætopterus, Phyllochaetopterus, Telepsaris, Polynoë, Acholoë, Tomopteris, Odontosyllis, Lepidonotus, Pionosyllis, Phyllodoce, Heterocirrus, Polyopthalamus?

Oligochæta

Lumbricus, Photodrilus, Allolobophora (Eisemia), Microscolex, Nonlea, Enchytræus, Octochætus.

Gephyrea (Sipunculus)

Hirudinea (Leeches)

Myzostomida (Myzostomus)

IX. Echinodermata