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Extracellular Matrix Stiffness Promotes Vascular Smooth Muscle Cell Calcification by Reducing The Levels of Nuclear Actin Monomers
Extracellular Matrix Stiffness Promotes Vascular Smooth Muscle Cell Calcification by Reducing The Levels of Nuclear Actin Monomers
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Length:
20 minutes
Released:
Jun 19, 2023
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.06.18.545506v1?rss=1
Authors: McNeill, M. C., Chee, F. L., Ebrhimighaei, R., Sala Newby, G. B., Newby, A. C., Hathway, T., Annaiah, A. S., Joseph, S., Carrabba, M., Bond, M.
Abstract:
1.0BackgroundVascular calcification (VC) is a prevalent independent risk factor for adverse cardiovascular events and is associated with diabetes, hypertension, chronic kidney disease, and atherosclerosis. However, the mechanisms regulating the osteogenic differentiation of vascular smooth muscle cells (VSMC) are not fully understood.
MethodsUsing hydrogels of tuneable stiffness and lysyl oxidase-mediated stiffening of human saphenous vein ex vivo,we investigated the role of extracellular matrix (ECM) stiffness in the regulation of VSMC calcification
ResultsWe demonstrate that increased ECM stiffness enhances VSMC osteogenic differentiation and VSMC calcification. We show that the effects of ECM stiffness are mediated via a reduction in the level of actin monomer within the nucleus. We show that in cells interacting with soft ECM, elevated levels of nuclear actin monomer repress osteogenic differentiation and calcification by repressing YAP-mediated activation of both TEA Domain transcription factor (TEAD) and RUNX Family Transcription factor 2 (RUNX2).
ConclusionThis work highlights for the first time the role of nuclear actin in mediating ECM stiffness-dependent VSMC calcification and the dual role of YAP-TEAD and YAP-RUNX2 transcriptional complexes.
2.0 GRAPHICAL ABSTRACT
O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=140 SRC="FIGDIR/small/545506v1_ufig1.gif" ALT="Figure 1" greater than
View larger version (24K):
org.highwire.dtl.DTLVardef@10b52aforg.highwire.dtl.DTLVardef@619f4borg.highwire.dtl.DTLVardef@1212f97org.highwire.dtl.DTLVardef@1bb9766_HPS_FORMAT_FIGEXP M_FIG C_FIG 9.0 HIGHLIGHTSO_LIIncreased ECM stiffness promotes VSMC calcification.
C_LIO_LIIncreased ECM stiffness reduces levels of nuclear actin monomer.
C_LIO_LIOn physiological soft ECM, high levels of nuclear actin monomer inhibits calcification by repressing YAP activation.
C_LIO_LIYAP activation promotes calcification by stimulating the activity of TEAD and RUNX2.
C_LI
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
http://biorxiv.org/cgi/content/short/2023.06.18.545506v1?rss=1
Authors: McNeill, M. C., Chee, F. L., Ebrhimighaei, R., Sala Newby, G. B., Newby, A. C., Hathway, T., Annaiah, A. S., Joseph, S., Carrabba, M., Bond, M.
Abstract:
1.0BackgroundVascular calcification (VC) is a prevalent independent risk factor for adverse cardiovascular events and is associated with diabetes, hypertension, chronic kidney disease, and atherosclerosis. However, the mechanisms regulating the osteogenic differentiation of vascular smooth muscle cells (VSMC) are not fully understood.
MethodsUsing hydrogels of tuneable stiffness and lysyl oxidase-mediated stiffening of human saphenous vein ex vivo,we investigated the role of extracellular matrix (ECM) stiffness in the regulation of VSMC calcification
ResultsWe demonstrate that increased ECM stiffness enhances VSMC osteogenic differentiation and VSMC calcification. We show that the effects of ECM stiffness are mediated via a reduction in the level of actin monomer within the nucleus. We show that in cells interacting with soft ECM, elevated levels of nuclear actin monomer repress osteogenic differentiation and calcification by repressing YAP-mediated activation of both TEA Domain transcription factor (TEAD) and RUNX Family Transcription factor 2 (RUNX2).
ConclusionThis work highlights for the first time the role of nuclear actin in mediating ECM stiffness-dependent VSMC calcification and the dual role of YAP-TEAD and YAP-RUNX2 transcriptional complexes.
2.0 GRAPHICAL ABSTRACT
O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=140 SRC="FIGDIR/small/545506v1_ufig1.gif" ALT="Figure 1" greater than
View larger version (24K):
org.highwire.dtl.DTLVardef@10b52aforg.highwire.dtl.DTLVardef@619f4borg.highwire.dtl.DTLVardef@1212f97org.highwire.dtl.DTLVardef@1bb9766_HPS_FORMAT_FIGEXP M_FIG C_FIG 9.0 HIGHLIGHTSO_LIIncreased ECM stiffness promotes VSMC calcification.
C_LIO_LIIncreased ECM stiffness reduces levels of nuclear actin monomer.
C_LIO_LIOn physiological soft ECM, high levels of nuclear actin monomer inhibits calcification by repressing YAP activation.
C_LIO_LIYAP activation promotes calcification by stimulating the activity of TEAD and RUNX2.
C_LI
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Jun 19, 2023
Format:
Podcast episode
Titles in the series (100)
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