Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2023.01.27.524347v1?rss=1

Authors: Yue, Y.

Abstract:
Tracing the differentiation of the hematopoietic progenitor cells in human blood, particularly with the scarcity in adults have been challenging owing to the technical limitations and sensitivity. Often, the use of classic and traditional surface markers lacks the sensitivity and specificity to clearly define the hematopoietic progenitors. With the evolution of single cell sequencing approaches, including single cell RNA sequencing (scRNA seq) technologies, this have enable the opportunities to study the heterogeneity and hierarchy of HPCs. Here, scRNA-seq was used to capture, identify and define the characterization of HPCs in adults peripheral blood. In addition, HPCs lineage were reaffirmed by unique makers and key factor genes. Several key factors, including NFE2, LYL1, MYB, and RUNX1 of myeloid and RUNX2, HOXA9, and BCL11A of lymphoid progenitors, were identified as major switches that controlled hematopoietic lineage formation. The reaffirmed lineage commitment also revealed hematopoiesis was a continuum procedure controlled by the activation and suppression of transcription factors, with identified boundary. With these information, an elaborate and comprehensive transcriptional atlas of all types of hematopoietic progenitors was then constructed. Importantly, this newly created atlas can enable accurate definition, hierarchy, and biological insights of hematopoietic progenitors for facilitating new research development in cell engineering, regenerative medicine, and disease treatment. Moreover, this open a wide variety of possibilities for artificial precise control progenitor cells differentiation and targeted treatment of immune disease, providing hope for untreatable diseases.

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