Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.12.19.521049v1?rss=1

Authors: Wichmann, C., Michanski, S., Henneck, T., Mukhopadhyay, M., Steyer, A. M., Gonzalez, P. A., Grewe, K., Ilgen, P., Gültas, M., Fornasiero, E. F., Jakobs, S., Möbius, W., Vogl, C., Pangrsic, T., Rizzoli, S. O.

Abstract:
In mammals, spatial orientation is synaptically-encoded by sensory hair cells of the vestibular labyrinth. Vestibular hair cells (VHCs) harbor synaptic ribbons at their presynaptic active zones (AZs), which play a critical role in molecular scaffolding and facilitate synaptic release and vesicular replenishment. With advancing age, the prevalence of vestibular deficits increases; yet, a direct link to the functional decline of VHC ribbon synapses remains to be demonstrated. To address this issue, we investigated the effects of aging on the ultrastructure of the ribbon-type AZs in murine utricles using various electron microscopic techniques and combined them with confocal and super-resolution light microscopy as well as metabolic imaging up to one year of age. In older animals, we detected predominantly in type I VHCs the formation of floating ribbon clusters. Our findings suggest that VHC ribbon-type AZs undergo dramatic structural alterations upon aging.

Copy rights belong to original authors. Visit the link for more info

Podcast created by Paper Player, LLC