Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.11.21.517317v1?rss=1

Authors: Ugolini, M., Kuznetsova, K., Oda, H., Kimura, H., Vastenhouw, N. L.

Abstract:
The localization of transcriptional activity in specialized transcription bodies is a hallmark of gene expression in eukaryotic cells, but it remains unclear if and how they affect gene expression. Here, we disrupted the formation of two prominent transcription bodies that mark the onset of zygotic transcription in zebrafish embryos and analyzed the effect on gene expression using enriched SLAM-Seq and live-cell imaging. We found that the disruption of transcription bodies results in the upregulation of genes that already have accessible chromatin and are poised to be transcribed when the two transcription bodies are present. Live-cell imaging suggests that the disruption of these transcription bodies enables poised genes to be transcribed in ectopic transcription bodies. Our results suggest that transcription bodies regulate transcription genome-wide by sequestering components of the transcriptional machinery.

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