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Initiation of fibronectin fibrillogenesis is an enzyme-dependent process
Initiation of fibronectin fibrillogenesis is an enzyme-dependent process
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Length:
20 minutes
Released:
Nov 17, 2022
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.11.16.516843v1?rss=1
Authors: Melamed, S., Zaffryar-Eilot, S., Nadjar-Boger, E., Aviram, R., Zhao, H., Yaseen-Badarne, W., Kalev-Altman, R., Sela-Donenfeld, D., Lewinson, O., Astrof, S., Hasson, P., Wolfenson, H.
Abstract:
Fibronectin fibrillogenesis and mechanosensing both depend on integrin-mediated force transmission to the extracellular-matrix. However, force transmission is in itself dependent on fibrillogenesis, and fibronectin fibrils are found in soft embryos where sufficient force cannot be applied, demonstrating that force cannot be the sole initiator of fibrillogenesis. Here we identify a novel nucleation step prior to force generation, driven by fibronectin oxidation mediated by lysyl-oxidase enzyme family members. This oxidation induces fibronectin clustering that promotes early adhesion, alters cellular response to soft matrices, and enhances force transmission to the matrix. In contrast, absence of fibronectin oxidation abrogates fibrillogenesis, perturbs cell-matrix adhesion, and compromises mechanosensation. Moreover, FN oxidation promotes cancer cells colony formation in soft agar as well as collective and single-cell migration. These results reveal a yet unidentified, force-independent enzyme-dependent mechanism that initiates fibronectin fibrillogenesis, establishing a critical step in cell adhesion and mechanosensing.
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http://biorxiv.org/cgi/content/short/2022.11.16.516843v1?rss=1
Authors: Melamed, S., Zaffryar-Eilot, S., Nadjar-Boger, E., Aviram, R., Zhao, H., Yaseen-Badarne, W., Kalev-Altman, R., Sela-Donenfeld, D., Lewinson, O., Astrof, S., Hasson, P., Wolfenson, H.
Abstract:
Fibronectin fibrillogenesis and mechanosensing both depend on integrin-mediated force transmission to the extracellular-matrix. However, force transmission is in itself dependent on fibrillogenesis, and fibronectin fibrils are found in soft embryos where sufficient force cannot be applied, demonstrating that force cannot be the sole initiator of fibrillogenesis. Here we identify a novel nucleation step prior to force generation, driven by fibronectin oxidation mediated by lysyl-oxidase enzyme family members. This oxidation induces fibronectin clustering that promotes early adhesion, alters cellular response to soft matrices, and enhances force transmission to the matrix. In contrast, absence of fibronectin oxidation abrogates fibrillogenesis, perturbs cell-matrix adhesion, and compromises mechanosensation. Moreover, FN oxidation promotes cancer cells colony formation in soft agar as well as collective and single-cell migration. These results reveal a yet unidentified, force-independent enzyme-dependent mechanism that initiates fibronectin fibrillogenesis, establishing a critical step in cell adhesion and mechanosensing.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Nov 17, 2022
Format:
Podcast episode
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