20 min listen
Two RhoGEF isoforms with distinct localisation act in concert to control asymmetric cell division
Two RhoGEF isoforms with distinct localisation act in concert to control asymmetric cell division
ratings:
Length:
20 minutes
Released:
Nov 6, 2022
Format:
Podcast episode
Description
Link to bioRxiv paper:
http://biorxiv.org/cgi/content/short/2022.11.06.515358v1?rss=1
Authors: Montembault, E., Deduyer, I., Claverie, M.-C., Bouit, L., Tourasse, N. J., Dupuy, D., McCusker, D., Royou, A.
Abstract:
Cytokinesis is essential for the partitioning of cellular contents into daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process as they drive the assembly and constriction of the contractile ring. However, how Rho1 is regulated to sustain efficient furrow ingression while maintaining correct furrow position remains poorly defined. Here, we show that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of cortical myosin. This enlarged zone of Rho1 activity becomes essential to adjust furrow position during ingression, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation patterns provides robustness to an essential process.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
http://biorxiv.org/cgi/content/short/2022.11.06.515358v1?rss=1
Authors: Montembault, E., Deduyer, I., Claverie, M.-C., Bouit, L., Tourasse, N. J., Dupuy, D., McCusker, D., Royou, A.
Abstract:
Cytokinesis is essential for the partitioning of cellular contents into daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process as they drive the assembly and constriction of the contractile ring. However, how Rho1 is regulated to sustain efficient furrow ingression while maintaining correct furrow position remains poorly defined. Here, we show that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of cortical myosin. This enlarged zone of Rho1 activity becomes essential to adjust furrow position during ingression, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation patterns provides robustness to an essential process.
Copy rights belong to original authors. Visit the link for more info
Podcast created by Paper Player, LLC
Released:
Nov 6, 2022
Format:
Podcast episode
Titles in the series (100)
FBXL4 suppresses mitophagy by restricting the accumulation of NIX and BNIP3 mitophagy receptors by PaperPlayer biorxiv cell biology