This podcast discusses an approach to the presentation of ambiguous genitalia by describing the typical sequence of sexual determination and differentiation, defining disorder (or difference) of sex development and ambiguous genitalia, generating a differential diagnosis for ambiguous genitalia, and outlining initial approach and management. This podcast was developed by Terra Morel, a third year medical student at the University of Alberta, and was created in collaboration with Dr. Elizabeth Rosolowsky, a pediatric endocrinologist at the University of Alberta.