Stereotaxic Brain Atlas of the Egyptian Fruit Bat

By Raya Eilam-Altstadter, Liora Las, Menno Witter and Nachum Ulanovsky

The Stereotaxic Brain Atlas of the Egyptian Fruit Bat provides the first stereotaxic atlas of the brain of the Egyptian fruit bat (Rousettus aegyptiacus), an emerging model in neuroscience. This atlas contains coronal brain sections stained with cresyl violet (Nissl), AChE, and Parvalbumin – all stereotaxically calibrated. It will serve the needs of any neuroscientist who wishes to work with these bats – allowing to precisely target specific brain areas for electrophysiology, optogenetics, pharmacology, and lesioning. More broadly, this atlas will be useful to all neuroscientists working with bats, as it delineates many brain regions that were not delineated so far in any bat species. Finally, this atlas will provide a useful resource for researchers interested in comparative neuroanatomy of the mammalian brain.

• Provides detailed and accurate stereotaxic coverage of the Egyptian fruit bat forebrain
• Contains 87 plates of coronal sections of adult Egyptian fruit bats, each with one Nissl-stained hemisphere and the other stained either for AChE or Parvalbumin
• Delineates brain structures in the bat brain
• Serves as an essential tool for directing electrophysiology, imaging, optogenetics, pharmacology and lesioning in Egyptian fruit bats, and bats more generally
• Provides a rich resource for comparative neuroanatomy of the mammalian brain

• Language: English
• Publisher: Academic Press
• Release date: Feb 15, 2022
• ISBN: 9780323996150

Raya Eilam-Altstadter

Dr. Eilam is head of the Immunohistochemistry Unit at the Weizmann Institute of Science, Israel. She is interested in brain anatomy and function in the normal and diseased brain, and across developmental stages. She studies brain neuroanatomy across multiple species, from Drosophila and zebrafish to rodents, bats, and humans.

Book preview

9780323996150_FC

Stereotaxic Brain Atlas of the Egyptian Fruit Bat

First Edition

Raya Eilam-Altstädter

Weizmann Institute of Science, Rehovot, Israel

Liora Las

Weizmann Institute of Science, Rehovot, Israel

Menno P. Witter

Norwegian University of Science and Technology, Trondheim, Norway

Nachum Ulanovsky

Weizmann Institute of Science, Rehovot, Israel

Table of Contents

Cover image

Title page

Copyright

Dedication

Acknowledgments

Methods

Abstract

Stereotaxic surgery

Histological methods

Stereotaxic reference system

Brain shape and brain weight

Delineation of structures

References

Index of structures

Index of abbreviations

Plates

Appendix 1

Appendix 2

Appendix 3

Appendix 4

Appendix 5

Appendix 6

Appendix 7

Appendix 8

Copyright

Academic Press is an imprint of Elsevier

125 London Wall, London EC2Y 5AS, United Kingdom

525 B Street, Suite 1650, San Diego, CA 92101, United States

50 Hampshire Street, 5th Floor, Cambridge, MA 02139, United States

The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, United Kingdom

Copyright © 2021 Elsevier Inc. All rights reserved.

No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Details on how to seek permission, further information about the Publisher’s permissions policies and our arrangements with organizations such as the Copyright Clearance Center and the Copyright Licensing Agency, can be found at our website: www.elsevier.com/permissions.

This book and the individual contributions contained in it are protected under copyright by the Publisher (other than as may be noted herein).

Notices

Knowledge and best practice in this field are constantly changing. As new research and experience broaden our understanding, changes in research methods, professional practices, or medical treatment may become necessary.

Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein. In using such information or methods they should be mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility.

To the fullest extent of the law, neither the Publisher nor the authors, contributors, or editors, assume any liability for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein.

Library of Congress Cataloging-in-Publication Data

A catalog record for this book is available from the Library of Congress

British Library Cataloguing-in-Publication Data

A catalogue record for this book is available from the British Library

ISBN 978-0-12-819297-9

For information on all Academic Press publications visit our website at https://www.elsevier.com/books-and-journals

Cover Design: Noa David Geller

Atlas Plates Graphical Design: Noa David Geller

Publisher: Nikki Levy

Acquisitions Editor: Natalie Farra

Editorial Project Manager: Sam W. Young

Production Project Manager: Niranjan Bhaskaran

Designer: Christian J. Bilbow

Typeset by SPi Global, India

fm01-9780323996129

Dedication

R. E.-A. dedicates this book to the memory of her beloved parents Lea and Walter Altstädter

Acknowledgments

We thank Eva Himovichi for help in initial histological processing and photography, and Mariana Melcón for initial rotation and alignment of the plates. Wairimu Gatome performed most of the tract-tracing injections in the hippocampal formation, on a large group of 17 bats, which served as a basis for delineating the hippocampal formation substructures; Heidi Kleven analyzed these track-tracing data and contributed to the delineations. Bente Jacobsen contributed to the preparation of the Appendixes. Saikat Ray performed tract-tracing injections in three bats and also stained prefrontal cortex sections with Nissl, AChE, WFS1, and NNat, and used these to delineate prefrontal subfields—cingulate, prelimbic, and infralimbic cortices; Efrat Solomon assisted him with processing these brains. We thank Inbal Biton for high-resolution MRI images taken from a separate individual bat, which aided in 3D reconstructions of the hippocampal formation (Appendix 6). We thank Michael Okun, Yonatan Katz, and Ilan